The Effect of pH on Enzymes

Enzymes are affected by pH in two ways. The first instance, electrostatic charges on groups at the enzymes active sites are modified, and, secondly those charges are responsible for holding the molecular shape and active configuration may be disrupted. Both of the above factors have significant influence on the catalytic properties of the enzymes.

These effects are illustrated using the enzyme Amylase and Invertase.

Experiments included:

• Effect of pH on Invertase activity

• Effect of extremes of pH on stability of invertase

• Effect of extremes of pH on stability of Amylase

Suitable for A-level or tertiary level students.

Kit contains enough equipment for 10 single students or 10 small groups.