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EDVOTEK Using CRISPR to Treat Cystic Fibrosis

by Edvotek

Product code: PP00052457

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In this experiment, students will simulate the use of CRISPR-Cas9 to target a genetic mutation found in a patient suffering from Cystic Fibrosis. Students will develop an understanding of guide RNA (gRNA) design, and use agarose gel electrophoresis to examine pre-prepared DNA samples after CRISPR treatment.

  • Group Size: For 8 Lab Groups
  • Storage: Room Temperature Stable. Storage of Ready-to-Load QuickStrip™ samples in the refrigerator is recommended
  • Time Required: Complete in 45 minutes

The kit includes:

  • Instructions,
  • Ready-to-Load QuickStrip™ DNA Samples
  • UltraSpec-Agarose™
  • Electrophoresis Buffer (50X)
  • 10X Gel Loading Solution,
  • FlashBlue™ DNA Stain

Additional required equipment & materials (not supplied with the kit):

  • DNA Electrophoresis tank and accessories including casting trays and special power supply
  • Micropipettes: 5-50 µl (Optional)
  • Balance
  • White Light Box
  • Microwave or Hot Plate

*PLEASE NOTE: This kit has been developed for being suitable for use in education only and is not for diagnostic purposes*

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